The purpose of this study was to ´evaluate the effects of caffeine and calcium on the activities of the osteoblastic cell from mouse calvaria. The author cultured osteoblastic cells obtained from the mouse calvaria and were divided into three groups : the caffeine-treated, the calcium-treated and the combine-treated group.
In caffeine-treated group, the cell toxicity was measured by MTT assay at 1, 2 and 4 days after treatment of caffeine. In all groups, the densities of the mineralized bone nodules were measured by imaging analyzer after Von Kossa staining. The alkaline phosphotase MP) activities were measured at 2, 7, 14, 21 and 28 days and the interleukin-1,8 activities at 48 hours after treatment of caffeine and calcium. The measurements were statistically executed with ANOVA test and the results were as follows.
1. The cellular toxicity of the caffeine increased with the concentration of caffeine during the incubation period.
2. The maximum densities of mineralization were observed at 0.2 mM caffeine-treated group, 1.2 mM calcium-treated group, 0.1 mM caffeine and 1.8 mM calcium-treated group.
3. The activities of ALP were peaked at 14 days at calcium-treated group as no-treated. But, the activities of ALP
increased with concentrations of caffeine at caffeine-treated group. At combine-treated group, the act of ALP were
peaked at 24 days at 1.2 mM, 1.8 mM calcium-treated group, But decreased at 2.5 mM calcium-treated group.
4. The activites of the IL-1 a were increased significantly at 0.2 mM caffeine-treated group, 1.8 mM calcium-treated group
and 0.1 mlVI caffeine and 1.8 mM calcium-treated group. But, they were decreased at all groups of high concentration.
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